A client utilizes Jump Desktop on Mac to access their local Windows servers via RemoteDesktop protocol, port 3389. When trying to connect to their servers they would see the error:

There was an error connecting to the computer. Error Code: 2.50 (0x32)

The client is able to connect to remote/external Remote Desktop servers without issue. After working on the Windows hosts it appeared that they would accept normal Windows RDP connections, steering the issue back to the Mac that is connecting.

After some research it was found that somewhere (MacOS 15.4?) the network security settings for Jump Desktop were changed which prevented Jump Desktop from accessing local servers.

On MacOS, settings, privacy & security, local network, be sure to enable Jump Desktop.